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OJVRTM

Online Journal of Veterinary Research©

15 (1): 53-60, 2011. Redacted 2017.

Cultivation of Corynebacterium pseudotuberculosis in a continuous culture

 

K.M. Suleiman1, H. Boehnel2, S.H. Babiker3, A.Z.S.A. Zaki4

 

1,4 Dept. of Microbiol., F. of Vet. Med., University of Khartoum, Khartoum North, Post code: 13314, Sudan. 2 George-August-University, Tropentierhygiene, Kellnerweg 6, 37077 Goettingen, Germany. 3 Central Veterinary Research Laboratories, P. O. Pox 8067 Alamarat, Khartoum, Animal Resources Research Corporation, Sudan.

 

ABSTRACT

 

Suleiman KM, Boehnel H, Babiker SH, Zaki AZ., Cultivation of Corynebacterium pseudotuberculosis in a continuous culture, Onl J Vet Res,  Volume 15 (1): 53-60, 2011. The purpose of this study was to cultivate high yields of good quality antigen to control caseous lymphadenitis in sheep and goats. Corynebacterium pseudotuberculosis was successfully cultivated in a continuous system using the IBT bioreactor fermentation technology. The pH and temperature of fermentation, medium components and the dilution rate were optimized. The highest levels of production were obtained at a pH of 8.0, temperature 31° C and a dilution rate 0.02h-1 using a modified Burrell’s liquid medium.

 

Key words: Corynebacterium pseudotuberculosis, caseous lymphadenitis, fermenter,

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