©1996-2009
All Rights Reserved. Online Journal of Veterinary Research . You may not store these pages in any form except
for your own personal use. All other usage or distribution is illegal under
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Online Journal of Veterinary Research ©
Volume 5: 245-250, 2001.
SDS-PAGE proteins in Corynebacterium pseudotuberculosis
toxin (goat).
Mohan P1, Jayaprakasan V2, Punnoose
KT2, Mini M2
1Research
Associate, Project Directorate on Animal Disease Monitoring And Surveillance
(PD_ADMAS), IAH & VB Campus, Hebbal, Bangalore, 2Department
of Microbiology, College of Veterinary and Animal Sciences, Kerala
Agricultural University, Mannuthy, Thrissur, Kerala, INDIA.
SUMMARY
Mohan P, Jayaprakasan
V, Punnoose KT, Mini M., SDS-PAGE Proteins in Corynebacterium pseudotuberculosis
toxin (goat), Online J Vet Res
5: 245-250, 2001. Three
toxins were extracted from culture filtrate (CF), whole cell lysate (WCL) and sodium chloride extract (SCE) from Corynebacterium pseudotuberculosis
and analysed by SDS-PAGE. Protein fractions with
molecular masses (MM) of 8-200, 20-200 and 19-191KDa were isolated. Toxin
fractions from field isolates and reference strain did not differ; 31.6 and
68KDa bands were found in the 3 toxins. One fraction (MM 31.6KDa) was
identified as phospholipase D exotoxin
which is responsible for dermonecrotoxicity
(antigenic fraction).
KEY WORDS: Corynebacterium pseudotuberculosis;
toxin; SDS_PAGE; caprine
©1996-2009
All Rights Reserved. Online Journal of Veterinary Research . You may not store these pages in any form except
for your own personal use. All other usage or distribution is illegal under
international copyright treaties. Permission to use any of
these pages in any other way besides the before mentioned must be gained in
writing from the publisher. This article is exclusively copyrightedin its entirety to OJVR publications. This
article may be copied once but may not be, reproduced or re-transmitted without
the express permission of the editors.