©1996-2018. All Rights Reserved. Online Journal of
Veterinary Research . You may not store these pages in
any form except for your own personal use. All other usage or distribution is
illegal under international copyright treaties. Permission to
use any of these pages in any other way besides the before mentioned must be
gained in writing from the publisher. This article is exclusively
copyrighted in its entirety toOJVR.This article may
be copied once but may not be, reproduced or re-transmitted without the express
permission of the editors. This journal satisfies the refereeing requirements
(DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or
any pages linking to this page you must link directly to this page only here
rather than put up your own page.
OJVRTM
Online Journal of Veterinary Research©
Volume 17 (1): 26-31,
2013. Slightly Redacted 2017.
Pathological
changes in embryonated eggs infected by Coxiella burnetti.
Norina L2, Noordin
MM1, Sabri MY1, Goh MY1,
Zamri-Saad M1, Sarenasulastri
AB2,
Azizul MO2, Norsyahril
A2, Krishnan M2, Salmeah AR2,
Cheah CP2.
1Faculty of
Veterinary Medicine, Univerity Putra Malaysia 43400 Serdang, Selangor, 2 Malaysian Animal Health
Laboratory (MALH), Bukit Tengah 14000 Bukit Mertajam,
Penang
ABSTRACT
Norina
L, Noordin MM, Sabri MY,
Goh MY, Zamri-Saad M, Sarenasulastri
AB, Azizul MO, Norsyahril
A, Krishnan M, Salmeah AR, Cheah
CP., Pathological changes in embryonated eggs
infected by Coxiella burnetii, Onl J Vet Res., 17 (1): 26-31, 2013. Seven day old Specific Pathogen Free (SPF) embryonated egg were infected with
Coxiella burnetii at
serial dilutions. Pathological changes in infected embryos, re-isolation
capacity and detection by immunohistochemistry (IHC) were evaluated. Minced
organs were inoculated with 7 dilutions including controls to provide 15 SPF 7
day old eggs per group. Eggs were examined daily by candling. Eggs died on day
2 at dilution 10-4 and on day 4 at dilution 10-5. Death
was not observed in other eggs after day 4 until harvesting day. Albumin in
eggs infected with 10-4 dilution on day 5 appeared cloudy with small
spotted white foci attached to the chorion’s membrane with collapsed blood
vessels. For each dilution embryos showed severe haemorrhage with opacity on
both eyes. Yolk sacs stained with Gimenez revealed
red coccobacilli. All dilutions of the yolk sac induced positives using the IHC
which has rarely been used to confirm Coxiella in yolk
sac membrane.
Key words: Coxiella burnetii, IHC, SPF embryonated
egg, goat
FULL-TEXT
(SUBSCRIPTION OR PURCHASE TITLE $25USD)