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OJVRTM
Online Journal of Veterinary Research©
(Including Medical and Laboratory
Research)
Established 1994
ISSN 1328-925X
Volume 27 (10):570-582, 2023.
In vivo papyrus protein
and crab shell bone scaffold in dogs.
Khayreia Kadhim Habeeb1,
Bahaa F Hussein1, Muayad
A Hussein2
1Department(s)
of Anatomy, College of Veterinary Medicine, University of Baghdad, 2Medical Engineering, University
of Technology, Baghdad, Iraq
Habeeb KK, Hussein BF, Hussein MA., In vivo papyrus
protein and crab shell bone in dogs, Onl J Vet Res.,
27 (10):570-582, 2023. We describe
a porous biodegradable bone scaffold without stem cells, implanted in twelve
3-4 year adult mixed breed male dogs weighing ~15kg. Implant consisted of 20
wt.% gelatin from organic bone, 40wt.% dextrin, 20g Al-kharit
from papyrus [7], combined with 20g crab shells powder with ~30% protein, ~35% calcium
carbonate, ~32% chitin and cellulose-polymer 2% nitrogen, 3% phosphorus, 23% calcium
and 1.3% magnesium. The scaffold was cross-linked sterilized by 25Kgy gamma
radiation. Micro structure, particle size and crystalline properties were
determined by scanning electron microscopy sputtered coated with gold and wide-angle
X-ray diffraction. For scaffold implant, a 6 cm incision was made in the skin
at craniomedial surface over the radial bone on the proximal third of the left
forelimb. A 3 cm critical size defect was created on radial bone by sterile saw
for scaffold implant. Microscopy by 120 days showed bone with CaCO3 graphite
crystals at 200nm with macro-microspore 25μm-90μm calcium carbonate
microcrystal polygonal pores and completely replaced defect.
Key word: Al-kharit, Crab
Shell, Tissue Engineering, Bioceramic Scaffold, Dogs.
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