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OJVRTM
Online Journal of Veterinary Research©
Volume 21(5):251-258, 2017.
Prevalence of Cryptosporidium spp in
sheep in AL-Taji, Iraq
Mohammed T. S. Al-Zubaidi
College of
Veterinary Medicine - University of Baghdad
ABSTRACT
Mohammed T. S. Al-Zubaidi
Prevalence of Cryptosporidium spp in Sheep in AL-Taji, Iraq, Onl J Vet Res.,
21(5):251-258, 2017. Prevalence of
Cryptosporidium spp
oocysts in naturally infected sheep in Iraq is reported. Fecal samples (90)
from 1-3 y old sheep from Al- Taji, Iraq during
February and May 2016 were examined by lugol's
iodine, modified Ziehl-Neelsen stain and PCR.
Prevalence of Cryptosporidium spp
infection was ~49% (44/90) by PCR, ~38% (34/90) by Ziehl-Neelsen and only ~28% (25/90) by lugol's
iodine tests. Highest prevalence occurred in sheep ˂ 6 months old at ~70%
(14/20) and lowest in 25-36 month old ones at ~35% (8/23). About 58% (25/43) of
diarrheic sheep shed Cryptosporidium oocysts in feces. DNA extracted
with 7 cycles of freezing-thawing in liquid nitrogen yielded more DNA than 3
three cycles by deep freeze. Results suggest that the SSU rRNA–based
PCR detected Cryptosporidium oocysts in stool samples with high
sensitivity.
Keywords
: Cryptosporidium , Sheep, Diarrhea, PCR.