1994-2019. All Rights Reserved. Online Journal of Veterinary Research. You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR publications. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors.

OJVRTM

Online Journal of Veterinary Research©

Established 1994 
ISSN 1328-925X

 

Volume 22 (9):799-805, 2018.

A C57BL/6 mouse model for atopic dermatitis in dogs induced by Dermatophagoides farinae.

 

Talita de Cássia Borges1 DVM MSc, Cecília Ferreira Amaral Silva1 DVM,

Paulo Henrique de Sampaio da Silva1 DVM MSc, Márcio Botelho de Castro1 DVM PhD,

Andre Rodrigues da Cunha Barreto Vianna2 DVM PhD, Eduardo Maurício Mendes de Lima1 DVM PhD.

 

1Faculty of Agronomy and Veterinary Medicine, University of Brasília (UnB), ICC Sul, Campus Universitário Darcy Ribeiro, Cx. Postal, 4508, Brasília, DF 70910-970. 2Department of Veterinary Medicine, Federal University of Lavras (UFLA), Campus Universitário, 37.200-000 Lavras, MG, Brazil (UFLA), Minas Gerais, Brazil.

e-mail: limaemm@unb.br

ABSTRACT

 

Borges TC, Amaral Silva CF, Sampaio da Silva PH, Castro MB, Cunha Barreto Vianna AR, Mendes de Lima EM., A C57BL/6 mouse model for atopic dermatitis induced by Dermatophagoides farinae mites in dogs, Onl J Vet Res.,. 22 (9):799-805, 2018. Atopic dermatitis (AD) with itching and inflammation is the most common skin allergy in dogs.  We describe a mice model for AD. Three groups of 3 C57B1/6 mice were infested twice on skin with a dressing embedded with 0.1ml extract of Dermatophagoides farinae mites in 2, 3 or 4 cycles. Mice were infested on days 0, 3, 13 and 16 and rested between days 6 and 13. Controls were taped the same dressing with saline only. We only observed erythema, excoriation and pruritus in mice infested 4 cycles. In those infested 4 cycles we found hyperplasia (controls 6.03 ± 0.37mm), (infested 17.17 ± 3.93mm) and increased mast cells (controls 26 ± 1.04) (infested 137 ± 15.29). However, we found increased perivascular infiltrate in all mice infested by Dermatophagoides farinae. The C57BL/6 mice model appears to simulate canine atopic dermatitis using repeat dermal infestations with Dermatophagoides farinae.

 

Key words: murine model, C57Bl6, canine atopy, allergy..

MAIN

 

FULL-TEXT (SUBSCRIPTION OR PURCHASE TITLE $35USD)