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OJVRTM
Online Journal of Veterinary
Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 29 (11): 713-718, 2025.
Detection of canine distemper by PCR of genital, conjunctiva, urine and blood samples.
Amaral HA, Cortez A,
Richtzenhain
LJ , Funada
MR, Soares RM, Durigon
EL, Larsson MHMA.
School of
Veterinary Medicine and Animal Science and Institute of Biomedical Sciences,
University of São Paulo, Brazil.
ABSTRACT
Amaral HA, Cortez A, Richtzenhain
LJ, Funada MR, Soares
RM, Durigon EL, Larsson MHMA. Detection
of canine distemper by PCR of genital, conjunctiva, urine and blood samples.
Onl J Vet Res., 29 (11): 713-718, 2025. We describe method to detect
distemper virus in conjunctival and genital swabs, urine and peripheral blood
mononuclear cells by reverse-transcription–hemi-nested–polymerase chain
reaction (PCR). Samples were taken from 50 dogs with neurological signs over 3
years. Non-vaccinated dogs or those vaccinated more than 50 days before sample collection
were included to exclude false-positives. Fragments of nucleoprotein genes
canine distemper virus were detected by hemi-nested PCR in 43 (86%), genital (80%)
and conjunctival swabs (74%), urine (74% )and peripheral
blood mononuclear cells (68%). Conjunctival and genital swabs,
and urine samples provided high detection sensitivity enhanced by testing two
clinical samples especially in dogs that had not shown extra-neural signs or
those in the convalescent and late stage of canine distemper.
Key words: canine distemper; conjuctival swab;
dogs; genital swab; hemi-nested-PCR; RT-PCR; urine.
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