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OJVRTM
Online Journal of
Veterinary Research©
(Including
Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume
25 (9):666-671, 2021.
Detection of canine distemper virus in conjunctival,
genital, urine and blood samples
by hemi-nested PCR in dogs
with neurological signs.
Amaral1 HA,
Cortez1 A, Richtzenhain1
LJ, Funada2
MR, Soares1 RM, Durigon2 EL, Larsson1 MHMA.
1School of Veterinary Medicine and Animal Science,
University of São Paulo, Brazil; 2Institute of Biomedical Sciences,
University of São Paulo, Brazil.
ABSTRACT
Amaral H, Cortez A, Richtzenhain
LJ, Funada MR, Soares
RM, Durigon EL, Larsson MHMA. Detection of canine
distemper virus in conjunctival, genital, urine and blood samples by
hemi-nested PCR in dogs with neurological signs, Onl
J Vet Res., 25 (9):666-671, 2021. Canine distemper is highly contagious inducing severe
neurological signs in dogs. We report incidence of virus in conjunctival and
genital swabs, urine and peripheral blood mononuclear cells by hemi-nested RT-PCR
taken from 50 dogs with neurological signs presented at the Veterinary Hospital,
School of Veterinary Medicine and
Animal Science, University of São Paulo, over 3 years. Only unvaccinated dogs or those vaccinated 50
days before sampling were tested to exclude false-positives due to vaccine
virus. Fragments of nucleoprotein genes canine distemper virus were detected by
hemi-nested PCR in 43 (86%) dogs. We found 40 (80%) in genital, 37 (74%) in
conjunctiva swabs, 37 (74%) from urine and 33 (68%) in peripheral blood
mononuclear cells. We find that conjunctival and genital swabs,
and urine samples provided high detection sensitivity and convenient sampling. Detection
was boosted by testing two samples especially in dogs without extra-neural
signs or convalescent late stage of canine distemper.
Key words: canine distemper; conjuctival swab;
dogs; genital swab; hemi-nested-PCR; RT-PCR; urine.
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