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OJVRTM
Online Journal of Veterinary Research©
Volume 19(5): 331-343, 2015.
Microbial
population diversity and distribution in gastrointestinal tract of cattle
Amir Hossein Asgari Safdar
Baft Branch, Islamic Azad University,
Baft, Iran.
ABSTRACT
Asgari Safdar AH.,
Microbial population diversity and distribution in gastrointestinal tract of
cattle, Onl J Vet Res., 19(5):
331-343, 2015. Three
beef steers (837± 60 lbs) were fed a high energy diet
of 2.7 Mcal of ME/Kg dry matter
with a fiber to concentrate ratio of 40:60 according to NRC-1996. After dietary
adaption the animals were sacrificed and reticular, ventral, ventral blind,
dorsal and dorsal blind sacs, omasum, abomasal, proximal, distal small and large intestinal, cecal, and rectum samples were assessed for resident
microbial populations. T-RFLP analysis displayed significant changes in
microbial population structure and distribution within the rumen and
gastrointestinal tract. The T-RFLP profiles detected over 280 different
bacterial species within the rumen and gastrointestinal tract.
Key Words: Microbial,
PCR, cattle, gastrointestinal tract, molecular techniques.
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