1994-2019. All Rights
Reserved. Online Journal of Veterinary Research. You
may not store these pages in any form except for your own personal use. All
other usage or distribution is illegal under international copyright
treaties. Permission to use any of these pages in any
other way besides the before mentioned must be gained in writing from the
publisher. This article is exclusively copyrighted in its entirety
to OJVR publications. This article may be copied once but may not be,
reproduced or re-transmitted without the express permission of the editors.
OJVRTM
Online Journal of Veterinary Research©
Volume 22 (7):547-560,
2018.
Clinical pathology and immuno-histochemistry of mammary tumors from military/pet dogs in
Iraq.
Huda Hameed Alabbody1, Inam Jasim Lafta2*
1National Cancer Research Centre, University of Baghdad, Bab Al-Moatham, Baghdad, 2Zoonotic Diseases Unit,
College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.
ABSTRACT
Alabbody HH, Lafta IJ., Clinical
pathological and immuno-histochemistry of mammary
tumors from military/pet dogs in Iraq. Onl J Vet
Res., 22 (7):547-560, 2018. Authors
previously reported ~25% mammary tumors in dogs
in Iraq which they partly attribute to carcinogenic war materials such as TNT.
In our study, 10 virgin un-spayed military/pet bitches aged 5-15 years
presented April 2016 to December 2017 with abnormal masses in the abdomen
with painful oedema, swelling, anorexia, weight loss,
weakness and mild fever. Examination of regional lymph nodes and thoracic radiography confirmed metastasis. Tumors
were excised and determined to be mostly canine adenocarcinomas (CMT),
involving multiple glands, solid in texture, 5-15 cm in size, located mostly in
the inguinal mammary glands at stage T3: >5 cm.
Microscopy confirmed presence of
adenocarcinoma in 8 dogs and solid carcinoma in 2 with half of tumors being
grade III. Tumors had pleomorphic hyperchromatic cell nuclei in stroma,
epithelial cells of ducts with squamous metaplasia, fibrosis
and infiltrates of inflammatory cells in interstitial mammary tissue. In
papillary carcinomas, we also found papillary projections into glandular acini,
with proliferation of epithelial cells. Gross lesions appeared as
multiple ulcerated cranial abdominal masses with congestion and bloody edema. Expression of
oestrogen (ER) and progesterone receptors (PR), and proliferation marker
(Ki-67) were determined by immunohistochemistry. Samples were negative for ER
and PR except for one PR sample which stained strongly. This dog had a small
low grade tumor (3 cm) grade. Tumors from 7 dogs were strongly positive to
Ki-67 localized in the nuclei of cells, but 3 tumors stained negative or weak.
Follow up for 18
months, included health status, recurrence or metastasis. Only 3
dogs survived with others dying by 12 months.
Keywords: Mammary tumor,
dogs, histology, immunohistochemistry, ER, PR, Ki-67.
FULL-TEXT (SUBSCRIPTION OR PURCHASE
TITLE $25USD)