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OJVRTM
Online Journal of Veterinary Research©
Volume 24 (5):290-293,
2020.
Avian influenza H9N2 virus in lung and kidney of
broilers 3, 7, 11 and 15 days post inoculation.
Tavakkoli
Hadi
Department
of Avian Medicine, School of Veterinary Medicine, Shahid
Bahonar University of Kerman, Kerman, Iran
ABSTRACT
Hadi T., Replication of avian influenza H9N2 virus in lung and kidney of broilers
3, 7, 11 and 15 days post inoculation, Onl J Vet
Res., 24 (5):290-293, 2020. Avian influenza
is a contagious viral disease of wild and domestic birds.
Groups of 25 day-old commercial (AA plus) 30 day old AA plus broilers were challenged
intranasally allantoic
fluid with 106EID50/100µl H9N2 virus. Birds were tested for H9N2
antibodies in blood by hemagglutination inhibition
test and lung and kidney tissue by HT-PCR at 3, 7, 11 and 15 days after challenge.
Another 25 birds were used as unchallenged controls. Clinical signs were
monitored daily for the 15 days and at necropsy 5 chickens from challenge and
controls groups were randomly selected for pathology. In 2/5 (40%) lungs or 4/5
(80%) kidneys of infected chicks we found most viral RNA on day 7. Other days
varied 0/5 to 1/5 but mostly in kidneys (Table 2). Lungs had cleared by day 15.
Clinical signs in challenged chicks were conjunctivitis, depression, excessive
lacrimation, ruffled feathers, decreased feed consumption, coughing and
sneezing. Signs were apparent by day 3 but most obvious at Day 7 correlating
with viral loads. By day 11 clinical signs reduced and disappeared by day 15.
At necropsy we observed hemorrhage in small intestine, congestion of trachea,
air sacculitis and swollen kidneys.
Keywords: Avian
influenza, Broiler, H9N2, Low pathogenic avian influenza, RT-PCR.
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