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OJVRTM

Online Journal of Veterinary Research©

Volume 8:79-84, 2004. Extensively redacted 2018.


E. coli infection and antibiotic resistance in moribund and healthy broilers.

 

Ahmad MD, Rauf A, Rai MF.

 

Department of Microbiology, University of Veterinary & Animal Sciences, Lahore-54000 Pakistan

 

ABSTRACT

 

Ahmad MD, Rauf A, Rai MF., E. coli infection and antibiotic resistance in moribund and healthy broilers, Onl J Vet Res., 8:79-84, 2004. Incidence of Escherichia coli infection and antibiotic resistance from moribund and healthy broilers at slaughter is reported. Of 103 strains of E. coli, 52 were isolated from moribund birds submitted for postmortem examination and 51 from the intestinal contents of the healthy birds brought from different slaughter points. In vitro pathogenicity of E. coli was determined by growth characteristics of isolated strains on Congo-red medium. Pathogenic strains were identified by production of brick red colonies on media after 48h incubation at 37 oC. These isolates were tested for in vitro antibiotic susceptibility by disc diffusion. In  moribund birds, we found prevalence for pathogenic Escherichia coli to be 86.53% compared with 13.46% for non-pathogenic strains. All strains tested were sensitive to enorfloxacin with an inhibitory zone of 2.11 ± 0.20 cm. Norfloxacin was the second antibiotic of choice (2.11 ± 0.9 cm). The majority of isolates were highly resistant to erythromycin and trimethoprim.

 

 

 

 

 

 

 

 

KEY WORDS: Escherichia coli, postmortem, slaughter age, broilers, antibiotic resistance


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