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OJVRTM
Online Journal of Veterinary
Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 29 (5): 278-284, 2025.
Protein fractions
in egg drop syndrome by electrophoresis
MH
Rasool, SU Rahman, MK Mansoor,
M Siddique.
Molecular
Virology Laboratory, Department of Veterinary Microbiology, University
of Agriculture, Faisalabad, Pakistan
Rasool MH, Rahman SU, Mansoor
MK, Siddique M., Protein fractions in egg drop syndrome by electrophoresis, Onl J Vet Res., 29 (5): 278-284, 2025. Six
isolates of egg drop syndrome (EDS) virus were recovered from 5 outbreaks in
layers. To induce infection, infected eggs were fed to susceptible layer hens.
Eggs from these birds were then were washed and cloacal tissues, oviducts and
spleens were collected, processed and inoculated in 11 days old duck embryos.
The presence of virus in harvested allanto-amniotic
fluid was checked by spot and microhaemagglutination
tests and confirmed by haemagglutionation inhibition
and agar gel precipitation tests. The EDS virus grew well in duck embryos but
agglutinated only avian, not mammalian red blood cells. These isolates were
purified through velocity density gradient centrifugation. Protein
concentration was determined through Lowry method and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) was
conducted by loading 300µg protein concentration on 12.5% gel using discontinuous
buffer system. All the six isolates showed 13 polypeptides, which were
identical to those described in the referral EDS-76 virus (strain-127). The
molecular weights of the polypeptides ranged from 6.5 KDa
to 126 KDa.
Key
words: Egg Drop Syndrome, Haemagglutination, Oviduct,
Duck embryos, Sodium dodecyl sulphate polyacrylamide
gel electrophoresis, polypeptides.
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