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OJVRTM
Online Journal of Veterinary Research©
Volume 9 (1) : 12-18, 2005. Redacted 2018.
Isolation of egg drop syndrome
virus and protein fractionation analysis using sodium dodecyl sulphate polyacrylamide gel electrophoresis
Rasool MH, Rahman SU, Mansoor MK, Siddique M
Molecular Virology Laboratory Department of
Veterinary Microbiology, University of Agriculture, Faisalabad- 38040, Pakistan
ABSTRACT
Rasool MH,
Rahman SU, Mansoor MK, Siddique M., Isolation of Egg
Drop Syndrome Virus and Protein Fractionation Analysis using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis Onl J Vet Res., 9 (1) : 12-18, 2005.
Six isolates of egg drop syndrome (EDS) virus were recovered from 5 outbreaks
of EDS in commercial laying hens. Aberrant eggs were fed to susceptible laying
hens to induce infection and tissue samples were inoculated in 11 days old duck
embryos. Virus in harvested allanto-amniotic fluid
was verified by spot and micro hemaglutination inhibition
and agar gel precipitation tests. The EDS virus grew in duck embryo and
agglutinated avian but not mammalian red blood cells. Isolates were purified
through velocity density gradient centrifugation. Protein concentration was
determined by the Lowry method and sodium dodecyl sulphate
polyacrylamide gel electrophoresis (SDS-PAGE) was conducted by loading 300µg
protein concentration on 12.5% gel using discontinuous buffer system. The six
isolates showed 13 polypeptides, which were identical to those described in the
referral EDS-76 virus (strain-127). The molecular weights of the polypeptides
ranged from 6.5 KDa to 126 KDa.
KEY WORDS:
Egg Drop Syndrome, Haemagglutination, Oviduct, Duck
embryos, Sodium dodecyl sulphate polyacrylamide gel
electrophoresis, polypeptides.