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Online Journal of Veterinary Research©

Volume 24 (1):74-81, 2020.

Molecular characterization of Eimeria kofoidi from partridge Alectoris chukar

and effect of curcumin, silymarin and salinomycin on oocyst sporulation.

 

Reza Abbasia, Seyed Mostafa Razavia, Bahman Abdi-Hachesoob*, Saeed Nazifib, Jafar Jalaeic

 

aDepartment(s) of Pathobiology, bClinical and cBasic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

 

ABSTRACT

 

Abbasi R, Razavi SM, Abdi-Hachesoo B, Nazifi S, Jalaei J., Molecular characterization of Eimeria kofoidi from partridge Alectoris chukar and effect of curcumin, silymarin and salinomycin on oocyst sporulation, Onl J Vet Res., 24 (1):74-81, 2020. Field isolate of Eimeria from a natural coccidiosis outbreak in the chukar partridge (Alectoris chukar) from a gamebird farm was identified by morphology as Eimeria kofoidi. Genus-wide Eimeria PCR primers were used to amplify the internal transcribed spacer 1 (ITS-1) gene, resulting in a partial sequence of 18S rRNA and a complete sequence of ITS-1 of the isolate. The resulting phylogenetic tree of ITS-1 placed Eimeria kofoidi (MK158919) from chukar partridge closest to Eimeria spp. from chickens. We then tested curcumin, silymarin and salinomycin on sporulation of Eimeria kofoidi oocysts Silymarin had no significant effect on sporulation compared with controls group. Salinomycin reduced sporulation only ~15% at 1mg/ml (p<0.05) whereas 0.25-1mg/ml curcumin reduced (p<0.05) sporulation~42-51%.

 

Keywords: Chukar partridge; Eimeria Kofoidi; ITS1 phylogeny; curcumin; salinomycin; silimaryn; oocysts; sporulation.

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