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OJVRTM

 Online Journal of Veterinary Research © 

Volume 14 (2): 194-207, 2010. Extensively redacted 2017.


Effects of electrical stimulation and ageing on postmortem changes and meat quality in fat-tailed sheep

 

Abbasvali, M.1, Shekarforoush, S.S.2, Aminlari, M. 3

 

1DVM, 2PhD, Department of Food Hygiene and public health, 3PhD, Department of Biochemistry, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

 

ABSTRACT

 

Abbasvali M, Shekarforoush SS, Aminlari M, Effects of electrical stimulation and ageing on postmortem changes and meat quality in fat-tailed sheep. Onl J Vet Res,  14: (2): 194-207, 2010. Effect of different voltages of electrical stimulation (ES), rigor temperature and ageing on biochemical changes and meat quality of fat-tailed sheep carcasses are described. Male sheep were subjected to 100 (n= 3) or 150V (n=3), 60 second, fixed frequency of 50 Hz.  Controls were not treated. Animals were then slaughtered and processed meat kept at 25 °C for 4 h, and chilled at 6 °C for 14 days. For each carcass, pH, temperature, adenosine triphosphate (ATP) content, calpain, water holding capacity (WHC), muscle color (L*, a*, b* color values), free amino acid (FAA), myofibrillar fragmentation index (MFI) and myofibrils gel electrophoresis patterns of longissimus dorsi muscle were determined. ES accelerated glycolysis producing falls in pH 1st 6h postmortem with a gradual decline to 24 h. There was a simultaneous reduction in ATP. We found no differences in muscle temperature at any time suggesting that ES did not affect carcass cooling rate. We found a decline in calpain during the first 6h postmortem.  Muscles from ES carcasses had lower WHC than controls at 1st day storage. There were no differences in color. ES affected FAA and MFI improving proteolysis and tenderness. By SDS –PAGE we found no changes in myosin or actin. We find that ES affects PM changes and meat quality.

 

Keywords: Electrical stimulation; Tenderness; Fat tailed sheep; Calpain; Water holding capacity


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