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OJVRTM

Online Journal of Veterinary Research©

Volume 23 (1):94-101, 2019.


Effect of Enrofloxacin on E. coli, hatchability, omphalitis and residue in Ross egg yolk and chick tissue

 

Nibras N Abdulhamza and Orooba MS Ibrahim.

 

Department of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine, University of Baghdad, Iraq.

 

ABSTRACT

 

Abdulhamza NN, Ibrahim OMS., Effect of enrofloxacin on E. coli , hatchability, omphalitis and residue in Ross egg and chick tissue, Onl J Vet Res.,  23 (1):94-101. Effect of enrofloxacin on growth of pathogenic E. coli isolated from yolk sac, hatchability, omphalitis and residue in eggs and chick tissue is described. Minimum inhibitory concentrations (MIC) were determined by broth dilution assay for  Enrofloxacin 30% and Ciprofloxacin 20% (NCCLS 2007). Enrofloxacin inhibited E coli dose dependently 2µg/ml MIC 16mm to 10 µg/ml MIC 24mm. Groups of 15 fertile Ross eggs each were then dipped in 2, 4, 6, 8 and 10 µg/ml enrofloxacin for 3 h at 37C followed by immersion at 4Co to permeate antibiotic through egg shell pores by temperature differential. Residue of enrofloxacin in egg yolks and in excised muscle and liver tissue of born chicks was determined by agar well diffusion test. Untreated controls had hatchability of 73.3% and omphalitis in 37% eggs. Eggs treated with 8 or 10µg/ml enrofloxacin hatched 100% with no omphalitis. 2-4µg/ml enrofloxacin hatched only ~80% preventing omphalitis in ~75%. 10mg/ml in egg yolk declined to 0.2mg/ml by day 17. We found no enrofloxacin in muscle or liver of 7 day chicks.

 

Keyword: Enrofloxacin, hatchability, omphalitis, E .coli, dipping.