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OJVRTM
Online Journal of Veterinary Research©
Volume 8:22-32, 2004, Redacted 2018.
Peptide AS-48 (Enterococcus faecalis) for prevention and treatment of mastitis
in dairy cows
Davidse EKa, Balla Ea, Holzapfel WHb, Muller CJCc, Cloete SWPc,
Dicks LMTa
aDepartment of Microbiology, Stellenbosch
University, 7600 Stellenbosch, South Africa, bInstitute
of Hygiene and Toxicology, Federal Research Centre for Nutrition, Haid-und-Neu-Str. 9, 76131,
Karlsruhe, Germany, cDepartment of
Agriculture, Private Bag X1, 7607 Elsenburg, South
Africa.
ABSTRACT
Davidse EK, Balla E, Holzapfel WH, Muller CJC, Cloete
SWP, Peptide AS-48 (Enterococcus faecalis) for prevention and treatment of mastitis in dairy
cows, Onl J Vet Res., 8:22-32, 2004. Peptide
AS-48, produced by Enterococcus faecalis FAIRE 92, inhibited the growth of a Staphylococcus aureus strain isolated
from mastitic milk. Peptide AS-48 was isolated from
the cell-free supernatant by using a combination of Triton X-114 phase
partitioning and cation exchange chromatography. The partially purified peptide
was liposome-encapsulated at a yield of 400 AU (arbitrary units)/ml and
injected into infection-free quarters of healthy Holstein cows. These quarters
were then infected by injecting 2 ml of the S. aureus pathogen (3.3 x 103 cfu/ml) through the teat canals. Control udders not
pre-treated with AS-48 were also injected with the same level of S. aureus. From the second day after
milking to day 7, the somatic cell count (SCC) in milk from udders that have
been pre-treated with liposome-encapsulated peptide AS-48 decreased by 60%. The
viable cell numbers of S. aureus in
milk from pre-treated udders remained more-or-less the same over the 7-day
period (1 x 102 cfu/ml), whereas the S.
aureus cell numbers in milk from untreated udders increased to 8 x 102
cfu/ml. When S. aureus-infected udders with a
SCC higher than 5 x 105/ml were injected together with
liposome-encapsulated peptide AS-48 (6 400 AU/ml), the SCC in milk from these
animals decreased by ca. 85% and the number of viable S. aureus by ca. 99%. Streptococcus
agalactiae and Streptococcus dysgalactiae, isolated from mastitic
milk, were also inhibited in in
vitro tests, but not Escherichia
coli.
KEYWORDS: Mastitis, Treatment, Peptide AS-48