©1996-2015
All Rights Reserved. Online Journal of Veterinary Research . You may not
store these pages in any form
except for your own personal use. All other usage
or distribution is illegal under
international copyright treaties.
Permission to use any of these
pages in any other way besides
the before mentioned must be gained in writing from the publisher. This article is exclusively
copyrighted in its entirety
to OJVR. This article may be
copied once but may not
be, reproduced or re-transmitted without the express permission of the editors.
This journal satisfies the refereeing
requirements (DEST) for the
Higher Education Research Data Collection. Linking:To link to this page
or any pages
linking to this page you
must link directly to this page only
here rather than put up
your own page.
OJVRTM
Online Journal of Veterinary
Research©
Volume
19(3): 162-168, 2015
Effect
of age on the viability of pig intestinal explants: histology and ultrastructure.
Karina M Basso DVM MSc
, Ana Paula FRL
Bracarense DVM PhD.
Laboratory of Animal Pathology, Universidade Estadual de Londrina, Londrina,
Paraná, Brazil.
ABSTRACT
Basso KM, Bracarense
AP., Effect of age on the viability of pig intestinal explants: a histology and
ultrastructure, Onl J Vet Res., 19(3):
162-168, 2015. Intestinal
explants are a useful model for toxicological and pathological studies, but the
maintenance of tissue viability for longer periods of incubation remains
challenging. The aim of this study was to evaluate the effect of age on the
viability of intestinal explants. Jejunal explants
were obtained from 24- and 42-day-old piglets. The explants were incubated for
two or four hours and were then processed for histological analysis. The
explants from younger pigs showed minor histological changes, as assessed by
histological and morphometrical scores. Explants from
24-day-old pigs were incubated for four hours and subjected to ultrastructural
analysis to assess cell integrity. The plasma membranes, nuclei and organelles
of enterocytes were well preserved, and adsorptive endocytosis was observed. In
conclusion, the data indicates that the age of the donor influences explant
viability. The intestinal morphology of incubated explants from young donors
was better preserved than that of explants from older piglets.
KEY-WORDS: ex vivo, intestine, jejune,
swine
FULL-TEXT(SUBSCRIBERS)
OR PURCHASE ARTICLE