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OJVRTM
Online Journal of Veterinary Research©
Volume 19(10):
679-683, 2015.
Genomic DNA mycobacterium avium
tests from goat intestinal tissue.
Khalideh Azari1, Mehdi Golchin1,
Reza Kheirandish1 and Mojtaba Alimolaei1,2
1Faculty of Veterinary
Medicine, Shahid Bahonar
University of Kerman, 2Razi Vaccine and Serum Research Institute-
Kerman branch, Kerman, Iran
ABSTRACT
Azari K, Golchin M, Kheirandish
R, Alimolaei M., Genomic DNA Mycobacterium avium tests from goat intestinal tissue, Onl J Vet Res., 19(10): 679-683, 2015. Genomic DNA Mycobacterium avium
extraction stool kits were compared with phenol-chloroform-isoamyl alcohol-proteinase K (PCIA) and a new PCIA
detergent method. DNA was extracted from intestinal tissue of a goat suspected
of john’s disease and test results were evaluated spectrophotometrically for
concentration of DNA extracted and quality (260/280 nm ratio). Kits yielded higher and PCIA’s least DNA
quantity, whereas PCIA’s yielded maximum DNA purity, and kits, least purity.
Keywords: Mycobacterium avium, DNA extraction, Novel, Intestine.
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