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OJVRTM
Online Journal of
Veterinary Research©
Volume 11 (2): 1 - 11,
2007. Redacted extensively 2018.
Evaluation of excretory-secretory
(ES) products of Fasciola gigantica ES-F5 with a serum and milk ELISA
for diagnosis
of Fasciola gigantica
infection in lactating buffaloes
Ghazy AA*, Abdel-Rahman EH, Abd El-Aziz MM.
Department of Parasitology and Animal Diseases
, Veterinary Research Division, National Research Center, Post Box 12622, 33
El-Tahrir Street, Dokki,
Giza, Cairo, Egypt.
ABSTRACT
Ghazy AA, Abdel-Rahman EH, Abd El-Aziz MM., Evaluation of excretory-secretory (ES) products of Fasciola
gigantica ES-F5 serum and milk ELISA for diagnosis of
Fasciola gigantica
infection in lactating buffaloes, Onl J Vet Res., 11 (2) : 89 -100, 2007. Excretory-secretory
(ES) products of Fasciola gigantica have
been shown to be a source of potential immunodiagnostic antigens. Five
fractions of crude F. gigantica ES obtained by gel filtration
chromatography were evaluated. Fraction 5 (ES-F5) showed higher serodiagnostic
value by ELISA with diluted F. gigantica
positive buffalo sera. By SDS-PAGE ES-F5 revealed bands with molecular weights
30.0, 26.0, 22.5, 20.0, 17.0, 14.0 and 13.5 KDa with
isoelectric points (PIs) of 4.1, 4.9, 5.3, 6.0, 6.3 and 8.3. Immuno-reactive
bands of ES-F5
antigen were identified with F. gigantica
positive buffalo sera and de-fated skim milk by Western immunoblot. Three
immunogenic bands (30.0, 14.0 and 13.5 KDa) were
identified in positive sera and milk but a22.5 KDa
band was specifically identified by positive sera whereas a band of 17.0 KDa was exclusively detected by positive milk. We tested
serum and milk ELISA ES-F5 antigen on 270 liver fluke (F. gigantica) infected or non infected
lactating buffaloes. Serum-ELISA gave a diagnosis of 62.2% milk-ELISA 58.2% and
parasitological examination 48.2%. Milk and serum samples from buffaloes
infected by F. gigantica confirmed by
detection of eggs in faeces, were used to estimate
sensitivity of the ELISAs. Serum and milk ELISAs using ES-F5 antigen provided sensitivities
of 99.2 and 97.7%, respectively. Results suggest that ES-F5 antigen was able to diagnose F.
gigantica in lactating buffaloes.
Keywords: Fasciola gigantica;
Lactating buffaloes; ES-F5
antigen; Serum-ELISA; Milk-ELISA.
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