©2023-2035 All Rights Reserved. Online Journal of Veterinary Research.
You may not store these pages in any form except for your own personal use. All
other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the
before mentioned must be gained in writing from the publisher. This
article is exclusively copyrighted in its entirety to onlinejournals@gmail.com
publications.. This article may be copied once but may
not be, reproduced or re-transmitted without the
express permission of the editors. Linking: To link to this page or any pages
linking to this page you must link directly to this page only here rather than
put up your own page.
OJVRTM
Online Journal of Veterinary
Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume
29
(6): 377-385, 2025.
Diagnosis of Fasciola
gigantica by SDS-PAGE and ELISA in serum and milk
of lactating cattle.
Ghazy AA, Abdel-Rahman EH, Abd El-Aziz
MM
Veterinary
Research Division, National Research Center, Dokki, Giza,
Cairo, Egypt.
ABSTRACT
Ghazy AA, Abdel-Rahman EH, Abd
El-Aziz MM Diagnosis of Fasciola gigantica
by ES-F5 and ELISA in serum and milk in lactating cattle., Onl J Vet Res.,, 29 (6): 377-385, 2025. Excretions
from Fasciola gigantica
are immunodiagnostic
antigens but require purification. We isolated 5 fractions by gel filtration
chromatography on Sephacryl S-200 column. Two fold
serially diluted F. gigantica positive buffalo
sera SDS-PAGE electrophoresis profile yielded molecular weights KDa of 30.0 with isoelectric point (PSI) of 4.1, 26.0
PSI 4.9, 22.5 PSI 5.3, 20.0 PSI 6.0,
17.0 PSI 6.3, 14.0 PSI and 13.5 KDa. WE isolated immunoreactive
bands of antigen in sera and defatted skim milk by Western immunoblot of 30.0,
14.0 and 13.5 KDa A 22.5 KDa band identified positive sera whereas 17.0 KDa occurred in milk. Using this method we diagnosed 270
liver fluke in infected and non infected lactating
buffaloes confirmed by fecal egg tests. Serum-ELISA yielded diagnostic value of
62.2% milk-ELISA 58.2% and fecal tests 48.2%. Milk and serum from F. gigantica infected cattle with fecal eggs yielded serum sensitivity
99.2% and milk ELISA 97.7%.
Keywords: Fasciola
gigantica; Lactating buffaloes; ES-F5 antigen; Serum-ELISA;
Milk-ELISA.
FULL-TEXT (SUBSCRIBE OR PURCHASE TITLE)