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OJVRTM

Online Journal of Veterinary Research©

 

Volume 19(2): 47-65, 2015.


Effect of diet on follicle size and expression of FSH receptor variants in ewes with progesterone implants. 

 

Amir Hossein Asgari Safdar1* and Vahid Ighani1

 

1Department of Animal Sciences, Faculty of Agriculture and Natural Resources, Science  and Research Branch, Islamic Azad University, Tehran, Iran.

 

ABSTRACT

 

Asgari Safdar AH, IghanimV., Effect of diet on follicle size and expression of FSH receptor variants in ewes with progesterone implants, Onl J Vet Res., 19(2): 47-65, 2015.  Flushing and expression of follicle stimulating hormone (FSH) receptor variants in Afshari Ewes fed different diets are described. Ninety three 1yr old Afshari ewes were implanted with a progesterone releasing devices (CIDR) to synchronize estrus and follicular development. Ewes were fed 6 combination diets with alfalfa, with or without prairie hay, or block and rolled corn supplements for 2 weeks. CIDR were removed and aspirated follicles were categorized medium sized 4 to 6 or larger > 6 mm. Total RNA was extracted from granulosa cells and reverse transcribed by qPCR of the resulting cDNA with primer sets for each variant of FSHR and LH receptor. Expression of FSHR-1 was different between medium and large follicles (P < 0.01) and ewes fed alfalfa hay (P<0.05). Overall mean expression of FSHR-3 was greater than FSHR-1 or FSHR-2 (P<0.01), but there was no difference in these values between medium or larger follicles, or between diets.  Concentrations of estradiol in follicular fluid were not affected by diet, follicle size nor  expression of lutenizing hormone receptor (LHR), suggesting that follicles developed equally. Live weights were different (P < 0.01) between diets but there were no differences in any group between non-esterified fatty acids concentrations, mean body condition (3.0) or lambing rates.  Results suggested that FSHR-1 was involved in later stages of follicular development, and possibly in follicle rescue. FSHR-3 was the most highly expressed FSHR and could be essential for antral follicle development. The findings show that a flushing response was not achieved in this study. Research is needed to determine how the initial energy status in ewes increases ovulation rate after energy supplementation (i.e. the flushing response).

 

Key Words: Flushing, Follicle Stimulating Hormone (FSH), Variants, Afshari ewes.


 

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