©1996-2018. All Rights Reserved.
Online Journal of Veterinary Research . You may not
store these pages in any form except for your own personal use. All other usage
or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the
before mentioned must be gained in writing from the publisher. This
article is exclusively copyrighted in its entirety to OJVR. This article may be
copied once but may not be, reproduced or re-transmitted without the express
permission of the editors. This journal satisfies the refereeing requirements
(DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or
any pages linking to this page you must link directly to this page only here
rather than put up your own page.
OJVRTM
Online Journal of
Veterinary Research©
Volume 18(4): 303-308, 2014. Redacted
2017.
Detection of vanA
genotype in Enterococcus gallinarum isolated
from poultry meat samples
Setareh Ameri
1, Ashraf Mohabati Mobarez
2 , Reza Hosseini Doust
1*
1Dept of Microbiology, Islamic Azad University, Branch of
pharmaceutical Sciences, 2Dept
of Bacteriology,
Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
ABSTRACT
Ameri S, Mohabati Mobarez A, Hosseini Doust R., Detection of vanA
genotype in Enterococcus gallinarum isolated from
poultry meat samples, Onl J Vet Res., 18(4): 303-308, 2014.
Enterococcus gallinarum from fowl also colonizes
humans but rarely cause infection. E. gallinarum resides
in fowls digestive lumen and are intrinsically
resistant to low levels of vancomycin.
Occurrence and molecular characterization of vancomycin susceptibility
in E. gallinarum isolated from poultry meat
samples is reported. Seventy chicken and 30 turkey meat samples were screened
by enterococci contamination assay. Isolates were confirmed as E. gallinarum by biochemical standard tests and
characterized by an antibiotic susceptibility test. Agar dilution was used to determine minimum
inhibitory concentrations (MICs). The strains were subjected to genetic
analysis of vancomycin resistant gene using specific primer sets.
Vancomycin-resistant E. gallinarum (VREG) (3),
vancomycin-intermediate E. gallinarum (VIEG)
(1), and vancomycin-susceptible E. gallinarum (VSEG)
(1) were isolated. Vancomycin @ MIC≥4 µg/ml confirmed the vancomycin
resistant gene VanA
in all strains except VSEG. This may be a first report of E. gallinarum carrying vanA
gene in poultry meat. The vanA gene may be
taken up by E. gallinarum as a transferable
genetic element.
Keywords:
Enterococcus
gallinarum, vanA, poultry meat.
FULL-TEXT(SUBSCRIPTION
OR PURCHASE TITLE $25USD)