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OJVRTM
Online Journal of Veterinary Research©
Volume 17 (3): 137-142, 2013. Slight redaction 2017.

Dose dependent effect of ozone on viability of Giardia duodenalis cysts

 

Fadia AL-Kayat(1); M.A.AL-Zuhairy(2) and May Hameed Kawan(3)

 

Department(s) of 1Basic Sciences, 2Public Health and 3Parasitology, Baghdad University

 

SUMMARY

 

AL-Kayat F, AL-Zuhairy MA, Hameed Kawan M., Dose dependent effect of ozone on viability of Giardia duodenalis cysts, Onl J Vet Res.,  17 (3): 130-136, 2013. The effect of ozone as a disinfectant on Giardia duodenalis cysts was evaluated in de-chlorinated water at 25șC and pH 8.2. The viability of cysts was assessed microscopically with eosine stain. Ozone was bubbled into inactivation reactors to obtain 0.05, 0.1 and 0.2 mg/L concentrations. 2x104cyst/ml G. duodenalis cysts were added to each reactor including controls. After contact times of 1, 3, 5 and 10 min ozone was removed with sodium thiosulfate 10%. Results showed that Giardia duodenalis cysts were more resistant to lower ozone concentrations with less exposure time. However 98.5% inactivation occurred with 0.2 mg/L ozone for 5 min. The findings suggest that using these parameters, higher ozone concentrations and contact times are required than those previously reported.

 

Key words:Giardia duodenalis, ozone, vital dye

                                                                                 

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