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OJVRTM
Online Journal of Veterinary Research©
Volume 12 (1):7-13, 2008. Extensively
redacted 2018.
Shahbazkia HRa, Nazifi Sb*.
aDepartments of Biochemistry and bClinical
Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Shahbazkia HR, Nazifi
S., Glycated
haemoglobin (HbG) and plasma glucose in fat-tailed sheep and lamb, Onl J Vet Res 12 (1): 7-13, 2008. Glycated haemoglobin (HbG) concentration
is a retrospective measure of blood glucose level and is not affected by recent
stresses, food ingestion or exercise. HbG has been
determined in kestrels, mankhor, mouflon, aoudad,
deer, goat, sheep, dog, camel and horses but not in fat-tailed sheep. We report
glycated hemoglobin and plasma
glucose
in adult sheep and lambs. Jugular blood
of 50 sheep and 30 lambs was washed of red blood cells for hemolysate
and cation exchange chromatography for determination of HbG.
Glucose was measured from plasma. In adult sheep, plasma glucose was 69.2 ± 3.24 mg/dl and HbG 2.58 ± 0.26 % of total haemoglobin
but in lambs 84.8 ± 9.31 mg/dl and 3.17 ± 0.29 % of total hemoglobin. HbG% correlated with plasma glucose (r = 0.82, p<0.05). There was no difference between male
and female HbG (P>0.05). To determine
if low HbG values in sheep were due to reduced
erythrocyte permeability to sugar, hemolysates were
incubated in 70mg/dl glucose in sheep and 85mg/dl in lamb and subjected to
chromatography. We found that HbG did not increase in
glycosylated hemolysates of lambs but did in adult
sheep. Lower erythrocyte glucose permeability may account for lower HbG in adult sheep compared with lambs.
KEY WORDS: Glycated haemoglobin(HbG), Cation exchange chromatography, Iranian fat-tailed
sheep, Lamb, Plasma glucose, Erythrocyte permeability.
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