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OJVRTM
Online Journal of Veterinary
Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 29 (7): 410-416, 2025.
Shahbazkia HR, Nazifi S.
Departments of Biochemistry and Clinical Pathology, School of Veterinary
Medicine, Shiraz University, Iran.
Shahbazkia HR, Nazifi S, Glycated hemoglobin (HbG)
associated with glucose in sheep and lambs., Onl J Vet Res., 29 (7): 410-416, 2025. We report
association between glycated hemoglobin and plasma glucose in sheep and lambs.
Jugular blood was taken from 25 male and female sheep and 30 lambs. After
separation and washing of red blood cells, hemolysate
was subjected to cation exchange chromatography for glycated hemoglobin and
glucose was measured in plasma. In sheep, plasma glucose was 69.2 ± 3.24 mg/dl
and glycated hemoglobin 2.58 ± 0.26% of total hemoglobin whereas in lambs were
84.8 ± 9.31 mg/dl and 3.17 ± 0.29% with correlation (r = 0.82, p<0.05). To
determine if low glycated hemoglobin in was associated with reduced
permeability of erythrocytes to glucose, hemolysates
were incubated 70mg/dl glucose in sheep and 85mg/dl in lambs for
chromatography. Our results suggest glycated increased in sheep but not in lambs.
Key Words: Glycated haemoglobin(HbG), Cation exchange chromatography, Iranian fat-tailed
sheep, Lamb, Plasma glucose, Erythrocyte permeability.