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OJVRTM
Online Journal of Veterinary Research©
Volume 21(2):81-84, 2017
Contraceptive HD tablet light/dark transition
and stool test for anxiety in female Balb/C mice.
Samaneh Gorani1, Mohammad Mahdi Zangeneh2,
Akram Zangeneh3*, Rohalah
Moradi4
Department(s)
1Toxicology, Pharmaceutical Sciences, Islamic Azad University, Tehran,
2Clinical Sciences, 3Microbiology, Pathobiology &
Basic Sciences, Faculty of Veterinary Medicine, Razi
University, Kermanshah, 4Chemistry,
Payame Noor University, Tehran, Iran.
ABSTRACT
Gorani S, Zangeneh
MM, Zangeneh A, Moradi R., Contraceptive
HD tablet light/dark transition and stool test for anxiety in Balb/C female mice, Onl J Vet
Res., 21(2):81-84, 2017. Light/dark
transition and defecation tests were used to assess anxiety-like behavior in
female mice given contraceptive tablets. One group of 6 mice were
given 35 µg/kg HD tablet per-os and another 6 were
saline controls. The light/dark transition defecation test was done in a 21x42x25cm
cage partitioned by 2 same sized chambers with a door. One chamber was
illuminated brightly and the other was dark. For 15, 20, 25, and 30 days after
treatments, mice were allowed to roam freely between chambers with the door left open 600
seconds at a time. Three seconds after placing mice in the dark chamber, the
door was opened to allow free roaming between chambers. Time spent and number of
stools in each chamber were recorded. After each trial, both chambers
were cleaned with ethanol to inhibit bias based olfactory cues. Results suggest
that mice given contraceptive tended to stay in the light chamber longer and
defecated more in both chambers compared with saline controls. Results should
be viewed with caution as placebo tablet was not tested. Raw data provided.
Keywords: Contraceptive HD tablet, anxiety, Light/dark
Transition test.
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