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OJVRTM
Online Journal of Veterinary Research©
Volume 22 (2):
94-98, 2018.
Effect of season on morphology of hematopoietic tissue in carp (Cyprinus carpio) fish.
Amer M. Hussin
College of Veterinary Medicine. Baghdad University, Iraq
ABSTRACT
Hussin AM., Effect of
season on morphology of hematopoietic tissue in carp (Cyprinus
carpio) fish. Onl J Vet Res., 22 (2):
94-98, 2018. Kidneys were excised from carp during winter (n=8) and summer (n=8) to
determine effect of season on morphology of hematopoietic tissue. Samples were
fixed in 10% neutral buffered formalin for 2 days for routine histology stained
by hematoxylin, Eosin and PAS. Hematopoietic tissue was
mainly concentrated in the dorsal kidney but the
caudal kidney had smaller areas of hematopoietic cords or cell islands with
sinusoidal capillaries. A high number of mitotic
figures were noticed especially in summer time indicating active hemopoiesis with several cell lines wherein nuclei appeared
large, spherical, euchromatic, with 2-3 apparent
nucleoli. Different sized nuclei with nucleoli were present whereas cytoplasm
appeared undeveloped or incomplete. A great number of active macrophages were
clearly noticed. Large Proerythroblast Rubriblast with loose, lacy chromatin, observable nucleoli,
and basophilic blue stained cytoplasm was evident. Megakaryocytes with highly
lobulated nucleii were also present. The presence of large number of active macrophages indicates their
important role in the process of hemopoiesis. The
findings suggest that proliferation of pluripotent stem cells
was more evident in fish collected during summer compared with those taken at
winter. However, in all tissue samples, we found little cellular differentiation.
Key
words: Histology, Carp, Hemopoiesis. Summer, winter.