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OJVRTM
Online Journal of Veterinary Research©
(Including Medical and Laboratory
Research)
Established
1994
ISSN 1328-925X
Volume 27 (8): 484-490, 2023
Heterogenicity of Staphyloccocus aureus mastitis coagulase gene.
Zahra Esmailnezhad,
Masoud Haghkhah, Narjes Ghaffari.
School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
ABSTRACT
Esmailnezhad Z, Haghkhah
M, Ghaffari N., Heterogenicity of Staphyloccocus aureus mastitis coagulase gene, Onl J Vet Res., 27
(9): 484-490, 2023. Coagulase phenotypes can identify virulence of S. aureus genotype strains. We report coagulase gene isolated
from 40 mastitis S. aureus cows by routine biochemistry confirmed by PCR
to yield 1.3kb products. Amplification of coagulase gene from all isolates yielded
single bands of 700 (30%) and 900 bp (70%) exclusively
bovine, suggesting that humans do not contaminate cow mammary glands. Digestion
of coagulase gene PCR products with restriction endonuclease enzymes AluI and Hin61 yielded 3 different RFLP
profiles that indicated a low heterogeneity in coagulase gene.
Key words: Mastitis, staphylococcus,
coagulase, genes, heterogeneity.
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