---

©2025-2037 All Rights Reserved. Online Journal of Veterinary Research. You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to onlinejournals@gmail.com publications.. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. Linking: To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page.

---

OJVRTM

Online Journal of Veterinary Research©

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X

Volume 29 (11): 719-730, 2025.

---

IL-1β and IL-8 mRNA proteins in equine bronchoalveolar fluids from obstruction due to straw inhalation.

Sarli Giuseppe, Peli Angelo, Marrocco Romina, Zanotti Lucia, Ducci Alice, Isani Gloria, Alessandra Scagliarini, Sassi Francesco, Benazzi Cinzia, Cinotti Stefano, Pietra Marco.

Dipartimento di Scienze Mediche Veterinarie, Università di Bologna, Corpo Sanitario Veterinario dell’Esercito Italiano, Roma

ABSTRACT

Sarli G, Peli A, Marrocco R, Zanotti L, Ducci A, Isani G, Alessandra S, Sassi F, Benazzi C, Cinotti S, Pietra MIL-1β, IL-8 mRNA proteins and cells in bronchoalveolar fluids of horses exposed to hay., Onl J Vet Res., 29 (11): 719-730, 2025. We report IL-1β and IL-8 mRNA proteins by RT-PCR and western blot in broncho-alveolar lavage fluid (BALF) cytology in 8 horses during partial obstruction induced by straw inhalation. We used 4 susceptible horses fed on pasture as controls. Compared with controls, in those exposed to hay, within Compared with controls, in those exposed to hay, within the BALF neutrophils increased 61-83% and epithelial cells 63-71% whereas lymphocytes declined 21-45% (P < 0.05). BALF neutrophils increased 61-83% and epithelial cells 63-71% whereas lymphocytes declined 21-45% (P < 0.05). In BALF horses increases (P < 0.05) in IL-1β mRNA protein preceded increase of IL-8. Increased IL-1β protein, but not mRNA, correlated (P < 0.05) with BALF neutrophilia. Increases in IL-8 mRNA and protein were transient, but protein peak at day 8 followed BALF neutrophilia peak. The increase of IL-1β, paralleled by neutrophil and epithelial cell accumulation in the BALF, is indicative of the active production of the cytokine by these cells. This reinforces the pivotal role of cells other than the macrophages in driving airway inflammation during RAO.

Key-words: IL-1β, IL-8 mRNA, proteins, Lung fluids, Equine.