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OJVRTM
Online Journal of Veterinary
Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 29 (11): 719-728,
2025.
IL-1β and IL-8 mRNA proteins in equine bronchoalveolar
fluids from obstruction due to straw inhalation.
Sarli Giuseppe, Peli Angelo, Marrocco
Romina, Zanotti Lucia, Ducci Alice, Isani Gloria, Alessandra Scagliarini, Sassi
Francesco, Benazzi Cinzia, Cinotti Stefano, Pietra Marco.
Dipartimento
di Scienze Mediche Veterinarie, Università di Bologna, Corpo
Sanitario Veterinario dell’Esercito Italiano, Roma
ABSTRACT
Sarli G, Peli A, Marrocco R, Zanotti L, Ducci A, Isani G, Alessandra S, Sassi F, Benazzi C, Cinotti S, Pietra M., IL-1β
and IL-8 mRNA proteins in equine bronchoalveolar
fluids from obstruction due to straw inhalation. ., Onl J Vet Res., 29 (11): 719-728,
2025. We report IL-1β and IL-8 mRNA proteins by RT-PCR and western blot in bronchoalveolar
lavage fluid (BALF) cytology in 8 horses during broncho
alveolar partial obstruction induced by straw inhalation. We used 4 susceptible
horses fed on pasture as controls. In BALF horses increases (P < 0.05) in
IL-1β mRNA protein preceded increase of IL-8. Increased IL-1β protein, but not mRNA, correlated (P < 0.05) with
BALF neutrophilia. Increased in IL-8
mRNA and protein were transient, but protein peak at day 8 followed BALF
neutrophilia peak. The increase of
IL-1β, paralleled by neutrophil and epithelial cell
accumulation in the BALF, is indicative of the active production of the
cytokine by these cells. This reinforces the pivotal role of cells other than
the macrophages in driving airway inflammation during RAO.
Key-words: IL-1β, IL-8 mRNA, proteins, Lung fluids, Equine.