1996-2019. All Rights Reserved. Online Journal
of Veterinary Research. You may not store these pages in any form except
for your own personal use. All other usage or distribution is illegal under
international copyright treaties. Permission to use any of these pages in
any other way besides the before mentioned must be gained in writing from the publisher. This article
is exclusively copyrighted in its entirety to OJVR. This article may be copied
once but may not be, reproduced or re-transmitted without the express
permission of the editors. This journal satisfies the refereeing requirements
(DEST) for the Higher Education Research Data Collection (Australia). Linking:
To link to this page or any pages linking to this page you must link directly
to this page only here rather than put up your own page
OJVRTM
Online
Journal of Veterinary Research©
Volume 21(4):141-145, 2017.
Density of connective and
white and red pulp tissue of spleens of adult working horses
determined
by stereological and morphometric methods.
Geórgia Dantas Roriz, André Rodrigues da
Cunha Barreto-Vianna, Anna Clara dos Santos,
Kaique Nogueira, Eduardo Maurício Mendes
de Lima.
Laboratory of Anatomy of Domestic
Animals, Faculty of Agronomy
and Veterinary Medicine, Brasilia,
DF, Brazil.
ABSTRACT
Roriz GD, Da Cunha Barreto-Vianna
AR, Dos Santos AC, Nogueira K, De Lima EMM., Density
of connective and white and red pulp tissue of spleens of adult working horses
determined by stereological and morphometric methods, Onl
J Vet Res., 21(4):141-145, 2017. The spleen is organized in arterial branches where
smaller arterioles end in a venous sinus system surrounded by a fibrous capsule
of connective tissue and trabeculae to sustain greater vascularization. Authors
quantify volume densities of connective and white and red pulp tissue of spleen
of 10 adult working horses by histomorphometric and
stereological techniques. Tissues were stained with hematoxylin & eosin, Picrosirius Red, Gomori and Azan
trichrome and then photomicrographed. Images fields
were captured by Olympus BX51 microscope analyzed with Image-Pro Plus® software
to measure and individualize tissues in 5 different fields per slide.
Furthermore, the STEPanizer® software was also used
for the point system analysis to compare the proportions of red and white pulps
and connective tissue. Normalized data was subjected to Pearson correlation at
P <0.05 significance. Mean + SD density of spleens was 0.99 g/ml ±
0.068. Connective tissue densities were 24.34±7.12 with Picrosirius
Red, 6.95 ± 3.78 with Gomori, and 8.41 ±
3.75% with Azan trichrome staining. However, with hematoxylin & eosin
stain, volume density of connective tissue was 8.64% ± 8.27, white pulp 3.70 ±
3.41%, and red pulp 66.66% ± 11.36. We assume picrosirius red stained
splenic musculature whereas Gomori and Azan Trichrome
differentiated the connective tissue. Immunohistochemical
analysis of spleen allowed us to determine percentage of connective tissue in
each spleen to establish a possible relationship with its contractile function.
Images of spleen with stains are shown.
Key
words: connective tissue, capsule, trabeculae, white pulp,
red pulp.
FULL-TEXT(SUBSCRIPTION OR PURCHASE
TITLE $25USD)