©1996-2018. All Rights Reserved. Online
Journal of Veterinary Research. You may not store these pages in any
form except for your own personal use. All other usage or distribution is
illegal under international copyright treaties. Permission to
use any of these pages in any other way besides the before mentioned must be
gained in writing from the publisher. This article is exclusively
copyrighted in its entirety to OJVR publications. This article may be copied
once but may not be, reproduced or re-transmitted without the express
permission of the editors.
OJVR TM
Online Journal of Veterinary Research
Volume 1:1-6, 2002. Redacted 2018.
Detection of sheep-goat interspecies
hybridization by analysis of satellite DNA
Isaäc J. Nijmana,
Martinus P. Hogendoornb,
Erik Gruysa, Gordon Luikartc,
Okan Ertugruld, Yo Zagdsurene, L.O. Ngeref, Noor Hasimag,
Georg Erhardth, Paolo Ajmone-Marsani
and Johannes A. Lenstraa
aFaculty of Veterinary
Medicine, Utrecht University Yalelaan 1, 3584 CL
Utrecht, The Netherlands bDelftsestraatweg 14, 2641
NB Pijnacker, The Netherlands. cLaboratoire
de Biologie des Populations d'Altitude,
Centre National de la Recherche Scientifique,
Unité Mixte de Recherche 5553, Université Joseph
Fourier, B.P. 53, F-38041 Grenoble Cedex 9, France. dAnkara Universitesi Veteriner Fakültesi, 06110 Diskapi, Ankara,
Turkey. eResearch Institute
of Animal Husbandry, Zaisan 210153, Ulaanbaatar,
Mongolia. fDept. Animal Science, University of Ibaden, Ibaden, Nigeria. gDept. Animal
Genetics and Cellular Biology, Institute Science Biology, University of Malaya,
50603 Kuala Lumpur, Malaysia. hDept.
Animal Breeding and Genetics, Justus-Liebig-Universität,
Ludwigstrasse 21 B, D-35390 Giessen, Germany iInstitute of Zootechnics,
Catholic University of S. Cuore, via Emilia Parmense, 84, I-29100 Piacenza, Italy.E
Email: J.A.Lenstra@vet.uu.nl
ABSTRACT
Nijman
IJ, Hogendoorn MP, Gruys
E, Luikart G, Ertugrul O, Zagdsuren Y, Ngere LO, Hasima N, Erhardt G, Ajmone-Marsan P, Lenstra JA. Detection of sheep-goat interspecies hybridization by analysis of
satellite DNA, Onl J Vet Res., 1:1-6, 2002. Sequence analysis of sheep and goat satellite II allowed
the identification of species-specific mutations that can be detected
conveniently by satellite restriction fragment length polymorphism (SFLP)
analysis. Restriction enzyme patterns did not depend on the sheep or goat breed
that were analyzed, while also ibex and bezoar, two wild goat species, had the
same patterns as domestic goat. The satellite II SFLP provides a rapid assay of
sheep-goat hybridization, which allowed the verification and disapproval,
respectively, of two cases of suspected goat-sheep hybridization.
KEYWORDS: Sheep, Goat, Hybrids, Species Origin,
Satellite DNA, PCR
FULL TEXT ARTICLE (SUBSCRIPTION OR
PURCHASE ARTICLE)