Volume
23 (6):606-614,
2019.
Egg shell hydroxyapatite implant for repair
of radial bone in rabbits.
Ali Ghazi
Atiyah¹, Nadia Hameed AL-Falahi².
¹Department(s)
of Surgery and Obstetrics, College(s) of Veterinary Medicine, University of
Tikrit. ²University of Baghdad,
Iraq.
ABSTRACT
Atiyah AG, AL-Falahi NH., Egg shell hydroxyapatite implant for repair of
radial bone in rabbits, Onl J Vet Res., 23 (6):606-614, 2019. Hydroxyapatite sintered with avian
eggshell powder was compressed for bone implant (HA) characterized by X-ray diffraction,
energy dispersive X-ray spectroscopy and scanning electron microscopy. HA consisted
of ~35% calcium, ~23% phosphorus (Ca/P 1.56) and ~42% oxygen similar to pure hydroxyapatite.
Then, in groups of 6-8 month ~1-1.8Kg male rabbits (n=15) a segmental defect of
5mm was created in the mid-shaft of the radius bone infilled by HA implant.
Controls (n=15) were not implanted. An external splint was applied on operated
limbs on rabbits for 4 weeks, sacrificed at 4, 8 and 12 weeks for bone samples.
Grossly, in implanted rabbits we found dense
white tissue from 4 weeks adhering to defective ends which then became bone
from the periphery and over the whole implant by 12 weeks. In contrast, in controls 4, 8 and 12 weeks after
surgery, we observed less (rounded) bone at both ends of the defect but the center
filled by fibrous connective tissue. Scanning
Electron Microscopy done before implant showed crystals as agglomerates
of spherical irregular particles with 10-50 μm
pores whereas 3 months we observed hematopoietic cells in direct contact with
the rough surface of the implant which enlarged together with dissolution of the
eHA particles. We found no infection or rejection in any rabbit but 2 controls had fractured ulnas supporting
body weight without splints.
Keywords: Eggshell, hydroxyapatite, bone implant, healing,
rabbit.
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