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OJVRTM
Online Journal of
Veterinary Research©
Volume 26 (7): 567-581, 2022.
Immuno clone for Streptococus agalactiae FbsA DNA mastitis vaccine.
Mohsen
Amini1, Azam Mokhtari*1,2, Ali Kadivar3, Somayeh
Shahrokh1
1Department of Pathobiology, 2 Zoonotic
Disease 3Clinical Sciences Faculty of Veterinary Medicine, Research Institute, Shahrekord University, Shahrekord,
Iran
ABSTRACT
Amini M, Mokhtari A, Kadivar
A, Shahrokh S., Immuno
clone for Streptococus agalactiae
FbsA DNA mastitis vaccine, Onl
J Vet Res., 26 (7): 567-581, 2022. FbsA virulence factor in Streptococcus agalactiae
induces mastitis in dairy cows. We cloned FbsA
gene from Streptococcus agalactiae for
immunization against mastitis. Sequence
of conserved region of gene was extracted from NCBI database and immunogenicity
of protein was evaluated. After alignment, ring-infected erythrocyte surface
antigen domain amino acids 89 to 292 were
selected with cleavage sites at BamH I ´5 and EcoR
I ´3 expressed without mismatch. Twelve amino acid epitopes within protein could
generate ~ 10 antibodies for humoral immunity. We predicted cell immunogenicity
finding ~25 epitopes recognized by B lymphocytes with 9 amino acids for antigen
binding. The desired fragment was cloned
into the plasmid pCDH-CMV-MCS-EF1-eGFP-T2A-Puro confirming recombinant plasmid
and cloning by restriction enzymes, colony PCR and sequencing.
Keywords: Mastitis, Streptococcus agalactiae,
FbsA, DNA.
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