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OJVRTM
Online Journal of Veterinary Research©
(Including Medical
and Laboratory Research)
Established
1994
ISSN 1328-925X
Volume 27 (12):664-669, 2023.
Processing M2e-HA2 influenza A
virus epitope from Escherichia coli for vaccine.
Masoud Moghadaszadeh,
Golchin M, Tavakkoli H, Mehdi Gol Reza Ghanbarpour.
School of Veterinary Medicine, Shahid Bahonar University of
Kerman, Kerman, Iran.
ABSTRACT
Moghadaszadeh M, Golchin M,
Tavakkoli H, Ghanbarpour
R., Processing M2e-HA2 influenza A
virus epitope from Escherichia coli
for vaccine, Onl J Vet Res., 27 (12): 664-669, 2023. High
mutation rate of influenza viruses, obfuscates production of efficient vaccines,
However, the viruses have conserved epitopes matrix-2
protein ectodomain M2e and HA2. We expressed and
purified M2e-HA2 peptides influenza genes in Escherichia coli
synthesized and cloned into pGS21vector. This construct was transferred into E.
coli BL21 (DE3) and induced by IPTG. Expression of recombinant peptide was
confirmed by western blot assay anti-GST monoclonal antibody. The expressed
peptide-GST was purified from bacterial lysate by IMAC chromatography.
Sequencing revealed that M2e-HA2 gene cloned in vector. SDS-PAGE
electrophoresis revealed prominent and Western blot single bands for potential
vaccine.
Key words: Cloning, Expression,
Purification, M2e-HA2, Escherichia coli, Influenza vaccine.
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