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OJVRTM
Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 29 (7): 394-401, 2025.
Diagnosis of bovine tuberculosis by G-interferon and
interleukin-2 receptor assay.
Ritelli M, Amadori M, Dondo A, Begni B, Zoppi S, Archetti IL
ABSTRACT
Ritelli M, Amadori M, Dondo A, Begni B, Zoppi S, Archetti IL. Diagnosis
of bovine tuberculosis by G-interferon
and interleukin-2 receptor assay, Onl J Vet Res 29 (7): 394-401, 2025. We validate dubious blood
mononuclear cells of g-interferon
for tuberculosis in cattle which are further tested for expression of
interleukin-2 receptor. 9.8% of 765 cattle reacted to g-interferon test with
1.2% dubious. Sixty four cattle had same results by both tests. For post mortem
tests, 80 of 95 cattle with 38 having TB lesions 36 were g-interferon positive
and 2 dubious. For IL-2 R assay, 33 with TB lesions scored positive, 1 negative
and 4 dubious. No g-interferon negative animal scored positive in the IL-2R
assay. Thirty-five out of 42
g-interferon positive animals were positive in IL-2R assay; 5 animals were
negative and 2 were scored dubious. Twenty g-interferon dubious animals were
scored positive and 10 negative in the IL-2R assay; only 4 were confirmed
dubious. As for SICT, 13 out of 16 SICT-positive animals were also positive in
the IL-2R assay. This way, both assays can be used sequentially on same blood
sample.
KEYWORDS: Cattle, Tuberculosis,
Diagnosis, g-interferon,
interleukin-2 receptor.