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OJVRTM
Online Journal of
Veterinary Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume
28(4): 252-260, 2024.
Diagnosis of
tuberculosis by g-interferon with interleukin-2 receptors assay.
Ritelli M, Amadori M, Dondo A, Begni B, Zoppi S, Archetti IL.
ABSTRACT
Ritelli M, Amadori M, Dondo A, Begni B, Zoppi S,
Archetti IL. Diagnosis of tuberculosis by g-interferon and interleukin-2 receptor assay, Onl J Vet Res., 28(4): 252-260, 2024. The g-interferon assay for bovine tuberculosis can be validated
further by expression of interleukin 2 receptor tests. Tuberculosis-positive
herds validated by PPD tuberculin, necropsy and bacteriology were subjected to
both tests. Seventy-five out of 765 samples were g-interferon positive (9.8%)
and 9 were dubious (1.18%). We found 73/565 g-interferon positive in one herd, 0/57
and 2/143 in other herds. Then we tested 78, 11 and 6 cattle in same herds by
the IL-2R assay and found 72 g-interferon positive, 14 negative and 9 dubious. Sixty
four cattle yielded the same result in both tests. Eighty of 95 cattle were
slaughtered for necropsy and we found 38 TB lesion-positive of which 36 were
g-interferon positive and 2 dubious. As for the IL-2 R assay, 33 were positive,
1 negative and 4 dubious. No g-interferon negative animal scored positive in
the IL-2R assay but 10 g-interferon positive and dubious samples scored
negative in the IL-2R assay; one being TB lesion-positive with highest
background expression of IL-2 receptor. Results of interleukin-2 receptor were highly
correlated (P < 0.001) with g-interferon assay. However
we found no increase in specificity.
KEYWORDS:
Cattle, Tuberculosis, Diagnosis, g-interferon, interleukin-2
receptor.