Volume 23
(1):66-74, 2019.
Prevalence of Cryptosporidium parvum,
hematology and immune markers in
hospitalized
children and pathogenicity in Swiss mice.
Hanan
Yousif Rasheed Alharby1, Thaer Abdulqader Salih Alalusi2
1Biology Department,
College of Education for Pure Sciences, University of Alanbar
and 2College of Science,
University of Alanbar, Iraq.
ABSTRACT
Rasheed Alharby HY, Salih Alalusi TA, Prevalence of Cryptosporidium parvum, hematology and immune markers in hospitalized
children and pathogenicity in Swiss mice, Onl J Vet
Res., 23 (1):66-74, 2019. Cryptosporidium parvum, was isolated from feces
of male and female children <10 years age. To assess pathology, the C parvum oocytes
were gavaged in groups of Swiss mice. Of 300 feces of
children tested, we detected C parvum in ~7%-13% feces by various methods with highest
incidence by ELISA (40 children). We found higher prevalence in males (15.6%)
than females (10.7%), aged 0-1y, ~29% and 6 to 9y 3.9%, from rural areas ~15%
and cities ~9%. Compared with non-infected controls, red blood cells were ~ 2%
lower, white cells 21% and platelets 17% higher, cholesterol 15%, triglyceride
21% and low and very low density lipoprotein 23-29% higher and protein 6%,
albumin 12% and globulin 4% lower. In infected children values for complement 4
were +36% higher, C3 27%, IgA 43% and interleukin L2, 33% higher. Significance for differences between infected
and non-infected data ranged P < 0.05 to <0.0001. In mice gavaged C parvum, we confirmed pathogenicity by observing infiltration
by neutrophils, vacoulation, hemorrhage, and
autolysis of structures with various stage of C parvum inside intestinal tissues. Our
results suggest that in young children, C
parvum mainly affected blood antibody immune
markers and lipids.
Keywords:
Cryptosporidium, children, hematology, immune markers, pathogenicity, mice.
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