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Online Journal of Veterinary Research © 

  15 (4): 366-380, 2011.  Redacted 2017.

Isolation and identification of a new isolate of avian infectious bronchitis virus IRFIBV32 and a study of its pathogenicity

 

Boroomand Z, (DVM)1, Razeghian I (DVM)2, Asasi K (DVM PhD)3, Mohammadi, A (DVM, PhD)4, Hoseini Noohadani, A. (DVM, PhD)5

 

1 Poultry Research Center, 2,4,5Department of Pathobiology 3Poultry Research Center, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

 

ABSTRACT

 

Boroomand Z, Razeghian I, Asasi K, Mohammadi A, Noohadani AH, Isolation and identification of a new isolate of avian infectious bronchitis virus IRFIBV32 and study of its pathogenicity, Online J Vet Res., 15 (4): 366-380, 2011. Infectious bronchitis (IB) is one of the most significant highly acute viral respiratory diseases of commercial chickens in the world. We isolated, identified and determined pathogenicity of an isolate of IB virus (IRFIBV32) from broiler chicken farms in Fars, Iran. Tracheal tissue samples of a field outbreak of respiratory disease in commercial broilers were processed for virus identification. The 5' part of the S1 gene of the spike protein was amplified and sequenced. The nucleotide sequence was found to have identity (95 %) with 793/B strains of avian infectious bronchitis virus. The isolate produced typical embryo lesions including curling and stunting, suggestive of infectious bronchitis virus infection after three passages. Infected allantoic fluids were tested by nested RT-PCR and then, ELD50 was calculated. Tracheal ciliary activity using carboniferous pigment granules in infected chicken inoculated by 105 ELD50/ml of the virus was evaluated. The results showed the isolate could induce ciliastasis. Experimentally affected chicks showed mild respiratory rales and difficult breathing. The most prominent gross lesions were observed in the trachea and kidneys of experimentally infected chickens.

 

Key words: Infectious bronchitis virus, isolation, pathogenicity, Iran

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