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OJVRTM
Online Journal of Veterinary Research©
Volume 21(8):484-489, 2017.
Effect
of royal jelly from nurse bees on In
vitro culture of spermatogonia stem cells from
pre-pubertal lambs
M Shahbazi,
AA Moghaddam*, P Rahimi-Feyli
Department of Clinical
Sciences, Faculty of Veterinary Medicine, Razi
University, Kermanshah, Iran.*Corresponding
author E-mail: moghaddam@razi.ac.ir
ABSTRACT
Shahbazi M, Moghaddam
AA, Rahimi-Feyli P., Effect of royal jelly from nurse
bees on In vitro culture of spermatogonia stem cells
from pre-pubertal lambs, Onl J Vet Res., 21(8):484-489, 2017. Royal jelly produced by young nurse bees
contains protein which stimulates cell growth and proliferation. Authors describe dose-response
effect of royal jelly (RJ) on proliferation of spermatogonia
stem cells (SSC) of pre-pubertal lambs. SSC were isolated from testes by 2-step
enzymatic digestion and cultured for 10 days in 5% fetal bovine serum in 0.313,
0.156 or 0.078 mg/ml RJ. Controls were FBS 5% without RJ. Glial cell-derived
neurotrophic factor was added as growth factor.
Media and colonies were refreshed, counted and measured at day 4, 7 and
10 of cultivation. SSC were identified by immuno-cytochemical
assay against protein gene product PGP9.5. There were no significant
differences in colony number or total surface area between any group.
Keywords:
Spermatogonial Stem
Cells, Royal Jelly, Fetal Bovine Serum, Cell culture