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OJVRTM

Online Journal of Veterinary Research©

Volume 23 (1):75-88, 2019.

Effect of light emitting diode (LED) irradiation on bovine In Vitro embryo growth.

 

Luis E. Vergara DMV Esp MSc1 , Mateus J. Sudano DMV Esp MSc PhD1, Jefferson V. Diniz DMV Esp MSc PhD*1, Pablo E. Ocampo-Ortiz DMV MSc1,

Andrés C. Moreno-Guio MV 2. Mauricio Montoya-Flórez1,3 DMV MSc PhD2, Fernanda da Cruz Landim DMV Esp MSc PhD1.

                       

1Faculty of Veterinary Medicine, São Paulo State University-UNESP, Botucatu, distrito de Rubião Jr Botucatu, SP 18618-000.  2Pedagogical and Technological University of Colombia, Tunja

3Colômbia Research Group in Veterinary Medicine and Husbandry - GIDIMEVETZ, Pedagogical and Technological University of Colombia, Tunja, Grupo de Pesquisa em Patologia Veterinária, Faculdade de Ciências Agropecuarias, Universidade de Caldas Manizales, COL. Faculty of Veterinary Medicine, Universidad Nacional de Colombia, Bogotá COL.*Corrresponding author: jeffvianadiniz@gmail.com, maomontoya53@yahoo.es

 

ABSTRACT

 

Vergara LE, Sudano MJ, Diniz  JD, Ocampo PE, Moreno-Guio A, Montoya-Flórez , Landim F. Effect of light emitting diode (LED) irradiation on In Vitro bovine embryos, Onl J Vet Res. 23 (1):75-88, 2019. Different tools have been proposed in order to increase in vitro bovine embryo production which remains low and sub lethal stressing to stimulate embryonic cells has been proposed. We describe effect of sub-lethal irradiation (LED) on bovine in vitro embryo production. One hundred and fifty zygotes were divided into 9 groups of 1 control, 4 irradiated with wavelengths of 633 nm (Red) and 4 with 820 nm (Infrared) in dosages equivalent to 10 J/cm². At different points, half of the zygotes underwent analysis through TUNEL assay to identify DNA fragmentation rates while the other half was frozen, thawed and submitted to the same analysis. Data was analyzed by ANOVA followed by Tukey’s test (P<0.05). We could not demonstrate a direct impact of LED sublethal stress over embryonic growth but in groups irradiated with infrared light we found a degree of protection for embryonic cells without affecting blastocyst production rate. Further studies with LED should attempt other wavelengths, filters, dosages and/or exposure times.

 

Key Words: DNA, irradiation, LED, sublethal stress, zygote.

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