MAIN


©1994-2018. All Rights Reserved. Online Journal of Veterinary Research. You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under International copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR publications. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editor


OJVR

 

Online Journal of Veterinary Research©

 

 Volume 14 (1):75-86, 2010. Redacted 2017.


Genetic diversity studies of long-term selected White Leghorn strains vis a vis a brown egger population by multi-locus DNA (RAPD) markers

 

Mahadeo Kumar1, SK Mishra2, VLK Prasad3, RP Sharma4 and BR Gupta5.

 

1Mahadeo Kumar, M.V.Sc., Scientist, Indian Institute of Toxicology Research, Lucknow 2 S.K. Mishra, Ph.D., Senior Scientist, Central Avian Research Institute, Izatnagar, Bareilly- 243122. 3 V.L.K Prasad, Ph.D., Professor, Department of Livestock Production and Management, College of Veterinary Science, Rajendranagar Hyderabad-500030 (A.P.), 4 R. P. Sharma, Ph.D., Director, Project Directorate on Poultry, Hyderabad, India- 500030.5 B.R Gupta, Ph.D., Department of Animal Genetics and Breeding, College of Veterinary Science, Hyderabad-500030 (A.P.)

 

ABSTRACT

 

Kumar M, Mishra SK, Prasad VLK, Sharma RP, Gupta BR, Genetic diversity studies of long-term selected White Leghorn strains vis a vis a brown egger population by multi-locus DNA (RAPD) markers, Onl J Vet Res, 14 (1):75-86, 2010. We analyzed genetic diversity of 5 egg-type closed chicken populations, viz. 4 White Leghorn (WLH) strains: IWI, IWH, IWF and IWD which had undergone selection for more than 20 generations, and one brown-egger strain: D. Red with variant geographic origin. A cumulating total of 145 randomly amplified polymorphic DNA (RAPD) markers generated from 10 most polymorphic primers, screened out of 42, were employed. The WLH strains exhibited distinct inter-population genetic differences as correlated to their origin and subsequent selection history. The intra-population genetic similarity ranged from 0.8484 to 0.9011 whereas inter-population genetic similarity ranged 0.5682 to 0.8854.  The coefficient of population-genetic differentiation (GST) was 0.422 which indicated higher contribution of within-population differences to genetic variation. A combined Dendrogram incorporating all the above strains in form of un-rooted tree was constructed, which revealed that the D. Red clustered distinctly away from the WLH strains, while displaying the highest genetic distance from IWD (0.5645). Within the WLH strains, the IWI and IWD were at maximum genetic distance (0.23) while the least (0.122) distance was recorded between IWF and IWD. It was concluded that differential genetic-homogeneity and distinct clustering patterns within egg type chicken strains consistent to their breeding and selection histories could be efficiently delineated by RAPD markers.

 

Key words:  Genetic diversity, polymorphism, RAPD, Long-term selection and WLH.


MAIN

 

FULL-TEXT(SUBSCRIPTION OR PURCHASE ARTICLE)