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OJVRTM
Online Journal of
Veterinary Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume
30 (7): 440-448, 2026.
Hemin-binding protein Leptospira antibody ELISA in bovine
serum
Subha Sivakolundu, SB Nagendrakumar, NA
Suneel, Dev Chandran, Manjula
Sritharan.
Department
of Animal Sciences, University of Hyderabad, Indian Immunologicals
Limited, Rakshapuram, Gachibowli,
Andhra Pradesh, India.
ABSTRACT
Sivakolundu S, Nagendrakumar SB, Suneel NA, Chandran
D, Sritharan M., Hemin-binding protein Leptospira antibody ELISA
in bovine serum, Onl J Vet Res., 30 (7): 440-448,
2026. Diagnosis by
bovine Leptospira
hemin-binding protein (HbpA) was tested to induce anti-HbpA antibodies in human. We used ELISA for detection of anti-HbpA antibodies in 176 bovine serum samples. Test was
validated by microscopic agglutination test (MAT) and leptospira
antigens LipL32 and LipL41. WE found a κ value of 0.920 for HbpA-ELISA vs LipL41-ELISA and 0.887 for HpbA-ELISA vs LipL32-ELISA suggesting correlation with HbpA standard leptospiral antigens.
The 3 antigen-based ELISA’s, showed 100% sensitivity but lower specificity (~ 63%)
compared with MAT. The lower specificity may have been due to a limited number
of serovars for MAT screening.
Keywords:
Leptospirosis, bovine, HbpA, LipL41, LipL32, ELISA.