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OJVRTM
Online Journal of Veterinary Research©
Volume 15(6): 476-484, 2011. Redacted 2018.
An ELISA for leptospiral
hemin-binding protein antibody in bovine serum.
Subha Sivakolundu (M.Sc.)1, S. B. Nagendrakumar
(M.V.Sc., PhD)2, N.A. Suneel (M.Sc.) 1,
Dev Chandran (PhD) 2, and Manjula Sritharan (PhD) 1*
1Department of Animal Sciences,
University of Hyderabad, Hyderabad 500046 Andhra Pradesh, India, 2Indian
Immunologicals Limited, Rakshapuram,
Gachibowli, Hyderabad 500032, Andhra Pradesh, India
ABSTRACT
Sivakolundu S, Nagendrakumar SB,
Suneel NA, Chandran D, Sritharan
M., ELISA for leptospiral hemin-binding protein
antibody in bovine serum, Onl J Vet Res., 15(6): 476-484, 2011. Leptospiral
hemin-binding protein (HbpA) is an iron-regulated
protein present in several pathogenic serovars but
absent in saprophytic leptospires. The diagnostic potential of HbpA for cattle was explored since it induces anti-HbpA antibodies in the serum of human patients with
leptospirosis. An ELISA-based assay for the detection of anti-HbpA antibodies in serum was used to screen 176 bovine
serum samples. The test was validated against the microscopic agglutination
test (MAT) and standard leptospiral antigens LipL32
and LipL41. The test showed a κ value of 0.920 for HbpA-ELISA
vs LipL41-ELISA and 0.887 for HpbA-ELISA vs
LipL32-ELISA indicating good agreement of HbpA with
standard leptospiral antigens.
The 3 antigen-based ELISA’s, showed 100% sensitivity but lower specificity (~
63%) compared with MAT. The lower specificity may have been due to a limited
number of serovars for MAT screening. Results suggest
that the described ELISA may have an advantage for detection of anti-leptospiral antibodies over MAT, which requires a large
number of live leptospiral serovars.
Keywords: Leptospirosis, bovine, HbpA, LipL41, LipL32, ELISA.
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