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Online Journal of Veterinary Research©
Volume 12 (2):23-32, 2008. Editors note: Redacted extensively
2018.
Ultrasonographic
and histopathological evaluation of hepatic lipidosis
in sheep with cobalt deficiency
El-Khodery SA1*, Hussein HS2, Metwally NS3, Nassif MN4, El-Boshy ME5,
Galbat SA6
1Department(s)
of Internal Medicine and infectious Diseases, 2,5Pathology, Faculty of Veterinary Medicine, Mansoura
University, 3Medicinal
chemistry, National research centre, Doki, Giza, 4Animal
Medicine, Faculty of Veterinary Medicine, Kafe
El-Sheikh University, 6Department of Parasitology and Animal Health,
National Research Centre, Giza 12311, Egypt.
ABSTRACT
El-Khodery SA, Hussein HS, Metwally
NS, Nassif MN, El-Boshy
ME, Galbat SA, Ultrasonographic and histopathological evaluation of hepatic
lipidosis in sheep with cobalt deficiency, Onl J Vet Res 12 (2 :23-32, 2008. We describe ultrasonographic and
histopathological changes in sheep with hepatic lipidosis
induced by cobalt deficiency. Thirty four sheep with illthrift
and anemia were studied and 23 were confirmed to have low blood cobalt (0.24 ± 0.09 mmol/l
and Vitamin B12 (0.15
±0.07 mg/l).
Ten normal sheep were used as controls. Sheep were examined with a real-time ultrasound
system with 5-MHz linear and convex transducers. Ultrasound guided liver
biopsies, blood and serum samples were obtained from each animal at
examination. Hepatic changes were
recorded in 21 (91.30%) sheep with cobalt deficiency. We found mild lipidosis in 5, moderate in 7, and severe lipidosis in 11
sheep. By ultrasonography, severe lipidosis
manifested as increased echogenicity of hepatic parenchyma. Focal hyperechioc lesions of different shape, size and position
were visualized in mild and moderate lipidosis. Liver
size increased significantly (P<0.05) in severely affected sheep compared
with controls. Histo-pathologically, macro-vacuolations, congestion of hepatic sinusoids, biliary
hyperplasia and infiltration of portal area with inflammatory cells and
connective tissues were found in moderate and severe lipidosis.
The findings suggest that ultrasonography can be used to diagnose lipidosis in sheep.
Key words: lipidosis,
sheep, Ultrasonography, Cobalt, pathology