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OJVRTM
Online Journal of Veterinary Research©
Volume 22 (2): 104-114, 2018.
Molecular characterization and
antimicrobial susceptibility of Listeria monocytogenes
isolated from human placentas and non-human samples.
Gany Kszaal
Atshan (MSc), Mawlood Abbas
Al-Graibawi (PhD).
Zoonotic Diseases Unit, College of Veterinary
Medicine, University of Baghdad, Iraq
ABSTRACT
Atshan GK, Al-Graibawi MA., Molecular
characterization and antimicrobial susceptibility of Listeria monocytogenes
isolated from human placentas and non-human samples, Onl
J Vet Res., 22 (2): 104-114, 2018. Fifty
human
placentas and 150 non-human samples from Thi
Qar, Iraq, were tested for Listeria
monocytogenes by inoculation in broth
cultured on Listeria selective Chromogenic and PALCAM agar. Isolates were confirmed by colony morphology, gram
stain, biochemistry and PCR’s to detect virulence
associated genes iap,
inlA and hlyA. A disc diffusion test
was performed to detect antimicrobial resistance. L. monocytogenes was
found in 7 (8.5%) placentas and 10 (6.6%) non-human samples.
PCR revealed that all isolates possessed the iap gene and 15 both inlA and hlyA
genes. Human
isolates exhibited high resistance to ceftazidime and penicillin G (100%) and
chloramphenicol (~86), but high sensitivity to tetracycline, streptomycin,
trimethoprim and norfloxacin (100%). Non-human
isolates exhibited high resistance to ceftazidime (~90%), penicillin G (~80%)
and chloramphenicol (~70%), but high sensitivity to norfloxacin,
streptomycin, tetracycline and trimethoprim (100%). Results suggest differences
in molecular and antimicrobial susceptibility between L.
monocytogenes isolated from human and non-human samples.
Keywords: Listeria monocytogenes,
Molecular characterization, iap
gene, inlA gene, hly
genes, PCR, Listeriosis.