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OJVRTM

Online Journal of Veterinary Research©

Volume 21(6):335-344, 2017.


Keratinase producing bacteria isolated from litter for digestion of keratin in feathers.

 

Somayeh Rahimnahal1. Amir Meimandipour2. Jamal Fayazi1*. Ali Asghar Karkhane2.

Mehdi Shamsara2. Mohammad Taghi Beigi Nassiri1

 

Department(s) 1Animal Science, Ramin Agriculture and Natural Resources University of Khuzestan, Mollasani, 2Animal Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

 

ABSTRACT

 

Rahimnahal S, Meimandipour A, Fayazi J, Karkhane AA, Shamsara M, Beigi Nassiri MT., Keratinase producing bacteria isolated from litter for digestion of feather keratin., Onl J Vet Res., 21(6):335-344, 2017. Keratin constitutes ~90% broiler feather weight which could be recycled into feed by keratinase producing microorganisms. Broiler litter samples were cultured in skim milk and keratine agar for keratinolytic microbes and isolated by microscope, biochemical characterization and molecular analysis. Keratin in feathers was extracted for feather meal broth spread on skim milk agar. Colonies which gave clear zones formed by hydrolysis of skim milk were re-cultured several times on fresh LB agar medium to produce purified single colonies. Of 25 strains isolated on skim milk agar Staphylococcus, Enterococcus, Bacillus safensis, B. Paenibacillus and Brevibacterium exhibited keranolytic activity. By 16S rRNA typing,  phylogenetic analysis and diameter on clear zone on keratin agar medium, Staphylococcus lentus SLKr1 and Bacillus safensis BSKr1 exhibited highest keratinolytic activity, high caseinase activity but were negative to amylase, lecithinase, cellulase and gelatinase. Bacillus safensis BSKr1 and Bacillus safensis BSKr2 also exhibited amylase, cellulase, caseinase and lecithinase activities. Results suggest that Staphylococcus lentus SLKr1 and Bacillus safensis BSKr1 possess high keratinolytic activities with potential use for digestion of feather keratin.

 

Key words: Feathers, Keratin, Keratinase. Broiler litter


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