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OJVRTM
Online Journal of Veterinary Research©
Volume 17 (6): 339-345, 2013. Redacted 2017.
Polymorphism of growth differentiation factor gene and
litter size in goats
Elham Javdan, Jamal Fayazi, Mohammad Taghi Baigi Nasiri,
Saleh Tabatabaei Vakili.
Department
of Animal Science, Ramin University of agriculture
and Natural Resources, Ahwaz, Iran
ABSTRACT
Javdan E, Fayazi J, Baigi Nasiri MT, Vakili ST., Polymorphism of growth differentiation factor
gene and litter size in goats, Onl J Vet Res., 17 (6): 339-345, 2013. Polymorphism of exon 2 of
growth differentiation factor (GDF9) gene was detected by PCR-SSCP in Najdi and Lori-Bakhtiari goats. 121 genomic DNA samples were used. DNA 295 bp
fragment from exon 2 of GDF9 gene was amplified by PCR with specific primers
and assayed by single stranded conformation polymorphism. AA, AB and
BB genotypes were detected with frequencies of 0.37, 0.423 and 0.207 in Najdi and 0.586, 0.173 and 0.241 in Lori-Bakhtiari goats, respectively. Heterozygosity was 0.424 and
0.172 in Najdi and Lori-Bakhtiari
goats. The results suggested that genotypes AA,
AB and BB detected in caprine GDF9 gene may show an association with allele B
at the exon 2 and litter size but further studies are required to validate the
polymorphisms.
KEY-WORDS : Goat Prolificacy,
Growth differentiation factor 9 gene, PCR-SSCP
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