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OJVRTM
Online Journal of Veterinary Research©
Established 1994
ISSN 1328-925X
Volume 23 (3):190-199, 2019.
Genetic diversity and DNA profiling of maize
varieties by ISSR-PCR.
Abdulmajed Abdulazez Hammadi al-Rawi, Mutaz Abduljabbar Farhan al-Fahdawi.
Al-Anbar University, College of Pure Science,
Department of Biology, Al-Anbar, Iraq.
ABSTRACT
Hammadi al-Rawi AA, Farhan al-Fahdawi MA., Genetic diversity and DNA profiling of maize varieties by ISSR-PCR, Onl J Vet Res., 23
(3):190-199, 2019. Maize (Zea mays L.) saccharata
(Z1), indurate (Z2), indentata (Z3), everta (Z4), tunicate (Z5) and Z. mays amylacea (Z6) cultivars used in Iraq were subjected to
10 ISSR DNA primers for genetic variation, relationship and dendogram
fingerprint. We found 65-90 micrograms DNA for each gram of leaf by CTAB with
purity range between 1.5-1.85 by PCR and electrophoresis. ISSR of 10 primers
amplified 366 and 198bp monomorphic 54.09% and 168bp were polymorphic (45.90%).
Our dendogram included one group of Z1, Z2 and
another minor Z5, Z4 and Z6 with Z3. Closest genetically by ISSR markers were
Z6 and Z3 and most remote Z1 and Z6. The results of the ISSR reactions
showed that in all 366 bands, the primer (IS-11) (62) had highest attachment. Genetic
distance amongst the six varieties ranged 0.09194 - 0.32896.
Key words: genetic diversity
, Zea mays L. , ISSR.
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